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Selection of the best combination of spectral filters for each biochemical parameter in an ensemble mode using ANN When the filter combination optimization for each parameter was performed, it was possible to define that the most frequent spectral filters for smoothing, baseline correction, normalization, and others were WDS, AsLS, SNV, and 1 st quadratic derivative, respectively (Table 3)

Furthermore, western blot was used to analyze the protein levels of representative senescent markers, including p16, p21, and p53

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