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Biosci Biotechnol Biochem 61(12):19771980 Van Der Werf P, Orlowski M, Meister A (1971) Enzymatic conversion of 5-oxo-L-proline (L-pyrrolidone carboxylate) to L-glutamate coupled with cleavage of adenosine triphosphate to adenosine diphosphate, a reaction in the -glutamyl cycle

For the observation of mitochondria generating ROS, the cells were incubated with HBSS (Sigma-Aldrich) containing 5 M MitoSOX for 10 min at 37 C in the dark and were then washed with PBS 3 times to remove any residual dye