Loukas M, Spentzouris G, Tubbs RS, Kapos T, Curry B: Ruggero Ferdinando Antonio Guiseppe Vincenzo Oddi
Biochimie 158, 208216
Antibodies and reagents The antibodies used in this study were listed as follows: anti-PGK1 (Proteintech, 17811-1-AP, 1:2000 dilution), anti-PGK1 (Santa Cruz, sc-130335, 1:1000 dilution), anti-Crotonyllysine (PTM BIO, PTM-501, 1:500 dilution), anti-Acetyllysine (PTM BIO, PTM-105, 1:500 dilution), anti-ECHS1 (Proteintech, 11305-1-AP, 1:500 dilution), anti-GAPDH (Proteintech, 60004-1-Ig, 1:1000 dilution), anti-VHL (Proteintech, 24756-1-AP, 1:500 dilution), anti-HIF-1 alpha (Proteintech, 20960-1-AP, 1:1000 dilution), anti-PCAF (Santa Cruz, sc-13124, 1:1000 dilution), anti-PDHK1 (Proteintech, 18262-1-AP, 1:2000 dilution), anti-phospho-PDHK1 (Thr338) (Signalway, 11596, 1:500 dilution), anti-Flag M2 (Sigma-Aldrich, F1804, 1:5000 dilution), anti-GST (Proteintech, 66001-2-Ig, 1:5000 dilution), anti-His (Proteintech, 66005-1-Ig, 1:5000 dilution), anti-HA (Proteintech, 51064-2-AP, 1:3000 dilution), anti-mouse IgG (H + L)/HRP (Jackson, 115-035-003, 1:10000 dilution), anti-rabbit IgG (H + L)/HRP (Jackson, 111-035-003,1:10000 dilution), anti-rabbit IgG (H + L)/FITC (ZSGB-Bio, ZF-0311, 1:200 dilution), anti-rabbit IgG (H + L)/TRITC (ZSGB-Bio, ZF-0316, 1:200 dilution), anti-mouse IgG (H + L)/FITC (ZSGB-Bio, ZF-0312, 1:200 dilution), anti-mouse IgG (H + L)/TRITC (ZSGB-Bio, ZF-0313, 1:200 dilution)
Strengthen, Strengthen, Strengthen You do not want the cycle of shin splints to continue, so make a strengthening routine a regular part of your training
