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Apply ice or a cold pack for one to two minutes before injection to numb the area and narrow capillaries

Sucrose fractionation Quadriceps (2.4 mg) pooled from five animals of each group were homogenized with a Dounce homogenizer in 1 mL polysome extraction buffer (PEB): 20 mM TrisHCl pH 7.5, 100 mM KCl, 5 mM MgCl 2 , 0.3 % NP-40, 60 U/mL RiboLock RNase Inhibitor (Thermo Fisher Scientific), 100 g/mL cycloheximide, 5 M FAD, cOmplete EDTA-free protease inhibitor cocktail and phosphatase inhibitor cocktail (Sigma-Aldrich)

She's reallly good with face fat transfer and cheek fat removal

The details of the treatment are shown in Table S1