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Monitoring recommendations: Baseline investigations: Full blood count, B12 level, folate, and intrinsic factor antibodies (if pernicious anaemia suspected) During initial treatment: Potassium monitoring in severe deficiency (risk of hypokalaemia) Follow-up: Repeat full blood count after 8 weeks to confirm haematological response Long-term: Annual review to ensure compliance with maintenance injections

All animal procedures were performed in adherence to the 3R principles (Replacement, Reduction, and Refinement)

Lots of processes in the body need B12 to operate effectively

If your protocol requires single-use vials or doses administered within 72 hours, sterile water is acceptable