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10.1128/AEM.66.4.1654-1661.2000 366

Reconstitution is the process of adding bacteriostatic water to lyophilized (freeze-dried) peptide powder to create an injectable solution

Lancet 406 , 15651586 (2025)

Afterward, the microbial species were rinsed two times with sterile PBS and then re-suspended in RPMI 1640 (for yeast) and MHB (for bacteria) medium (1.0 10 6 cells/mL)