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Interestingly, reduction of glutathione via RNAi treatment is reported to decrease daf-2 lifespan (Spanier et al., 2010), but in wild type animals has been found to cause a minor (47%) lifespan increase (Urban et al., 2017)

These findings are consistent with earlier randomised studies, such as the Physicians Health Study (345) and Womens Health Initiative dietary supplement trials (Ca/Vit D arms with cancer endpoints) (346), which similarly failed to demonstrate a protective effect against breast cancer development

Moreover, the levels of proteins associated with cell proliferation, migration, and angiogenesis (Slc38a2, Psph, Jagn1, and Rorb) were upregulated