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In patients with HS, splenectomy is the gold standard therapeutic option for severe hematologic phenotype, but indications and long-term cost-effectiveness in moderate to mild HS are not well established (51)

Captive animals were maintained under standard husbandry conditions (e.g., ad libitum food, absence of predators and competitors), which relax the constraints imposed by tradeoffs among competing functions

The key findings on selenium iodine interactions were: 1) Synergistic effects on nutrient accumulation: The combined application of selenium and iodine led to a significant increase in leaf iodine content, especially at higher selenium concentrations

The filtered supernatant was run over a protein A/G column (Pierce), followed by extensive washing with PBS and the antibodies were eluted with 18 mL 0.1 M glycine pH 2.5 directly captured in 2 mL neutralization buffer (1 M TRIS pH 8.7)