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Arabidopsis VPS35, a retromer component, is required for vacuolar protein sorting and involved in plant growth and leaf senescence

Trypsin, penicillin, streptomycin, ethanol, methanol, Tris-HCl, Tris-Base, Glycine, acrylamide/bisacrylamide (37:1, 2.6% crosslinker), sodium-dodecyl sulfate, ammonium persulfate, N,N, N,N-tetramethyl ethylenediamine (TEMED), resazurin sodium salt, lithium lactate, iodonitrotetrazolium chloride, -nicotinamide adenine dinucleotide (NAD), propidium iodide (PI), protease inhibitor cocktail, bovine serum albumin (BSA), Bradford reagent, 1-methoxy-5-methylphenazinium methyl sulfate (MPMS), caspase-3 colorimetric substrate (Ac-DEVD-pNA), HEPES, sodium chloride (NaCl), 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate (CHAPS), ethylenediaminetetraacetic acid (EDTA), glycerol and DL-dithiothreitol (DTT), iodonitrotetrazolium salt (INT), dipotassium phosphate (K 2 HPO 4 ), monobasic potassium phosphate (KH 2 PO 4 ), sodium thioglycolate (C 2 H 3 NaO 2 S), and sodium thionite (Na 2 S 2 O 4 ) were purchased from Sigma-Aldrich (Saint Louis, USA)

Coronaviruses are enveloped single-stranded positive-sense RNA viruses named after their crown-like appearance of their spike proteins on their surface (Singhal, 2020)

& Zhan, W