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In the following day, slices were washed (5 min at RT x 3 times) in PBS, mounted in Superfrost Plus Adhesion Microscope Slides (Thermo Scientific), baked at 60C for 30 min and post-fixed in pre-chilled 4% PFA in PBS (15 min at 4C)

Unfortunately, the positive effects of GHK-Cu decline with age, as its concentration in the body decreases over time

Evidence of a role for one-carbon metabolism in blood pressure: can B vitamin intervention address the genetic risk of hypertension owing to a common folate polymorphism