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doi:10.1016/j.ando.2019.09.004 Archontogeorgis K, Nena E, Papanas N, et al

And provide patients with education regarding safe use, storage, and disposal of opioids, and be aware of early warning signs of misuse

The reaction samples with a total volume of 50 L were prepared by mixing 0.3 L of diluted genomic DNA, 5.3 L of PCR-grade water, 12.5 L of 2 buffer, 5.0 L of a dNTP mix, and 0.7 L each of 10 mol/L forward and reverse primers