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Materials and methods Media and culture conditions Escherichia coli DH5 strains were grown at 37 C in LB medium (1% tryptone, 0.5% yeast extract, 1% NaCl w/v) supplied with 50 g/mL kanamycin or 100 g/mL ampicillin (Sigma-Aldrich, Missouri, USA) for cloning and plasmid propagation

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CRISPR Cas9 for Treatment and Control of Avian Viral Diseases: Challenges and Opportunities. World's Poultry Science Journal 80: 745765

The microbes in the gut break down EGCGs large polyphenol structures into smaller, active compounds, which can boost its effects throughout the body