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A putative model has been that the restriction of late gene activation may attribute to viral genome structure and viral DNA replication temporally remodels the chromatin structure, therefore relieving the restrain for the late gene expression and allowing the vPIC binds to the TATA box region of late gene promoter

Biomolecules 15 (4), 525

tumefaciens strain GV3101 carrying pYL156-RNA1 , pYL156-MEKK1 , pYL156-BAK1 / SERK4 , pYL156-CLA1 or pYL156-GFP (the vector control) was grown in LuriaBertani (LB) liquid medium containing 50 g ml 1 kanamycin and 25 g ml 1 gentamicin at 28 C for 48 h, and the culture was then transferred into fresh liquid LB medium containing 50 g ml 1 kanamycin, 25 g ml 1 gentamicin, 10 mM MES and 20 M acetosyringone for overnight incubation at 200 rpm at 28 C

The authors isolated lipid particles and identified 21 proteins, involved in fatty acid activation, lipid and sterol metabolism, and transport processes (Athenstaedt et al., 2006)