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The mRNA levels of CCL2 (C-C Motif Chemokine Ligand 2), CCL11 (C-C Motif Chemokine Ligand 11), CSF2 (Colony Stimulating Factor 2), CXCL10 (C-X-C Motif Chemokine Ligand 10), HGF (Hepatocyte Growth Factor), IL1RA (Interleukin 1 Receptor Antagonist), IL6 (Interleukin 6), IL10 (Interleukin 10), IL31 (Interleukin 31), LIF (LIF Interleukin 6 Family Cytokine), and NGF (Nerve Growth Factor) were quantified using specific primers in SYBR Green (Thermo Fisher) or TaqMan (Thermo Fisher) platforms following the manufacturers instructions

Most people benefit from: Weekly injections for overall wellness Bi-weekly injections for maintenance Twice weekly injections during illness, stress, or heavy travel Many patients pair vitamin C with B12, glutathione, or wellness IV drips for enhanced results

+ Case 3 - Acute Pain Control A 52-year-old female with past medical history of HTN, HLD, and OUD (tx with buprenorphine for the last 2 years) presents to the emergency department with right ankle pain after tripping on an icy step

Activation of mast cells releases histamine and mast cell proteases that may also damage intestinal barrier