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Seizure Score The rats were placed in a box where behavioral changes were monitored within a period of 20 min following PTZ injections and scored according to Racines stages scores (RSS) (Corda et al

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After taking an aliquot for protein estimation, worms were resuspended in 100 l of H 2 O and 230 l of Eluent A (95% acetonitrile, 0.1 mM ammonium acetate and 0.01% NH 4 OH) was added together with 1 l of 1 M chloropropamide as internal standard