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(6) When the clinical situation allows, repeating the test after the patient has stabilized shows whether the abnormality was passing or persistent

Real-time quantitative PCR (RT-qPCR) The total RNA was extracted from leukocytes using the QIAamp RNA Blood Kit (Qiagen, Hilden, Germany), and cDNA was produced with the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher, Waltham, MA) as previously described 18

Whit Cooney earned his RDN credential on 6/6 from the Commission on Dietetic Registration and started a new position as pediatric RDN at Childrens Healthcare of Atlanta Scottish Rite

Studies were included if a specific human milk fat globule membrane component (phospholipids, proteins, gangliosides, and cholesterol) was measured at two or more time points during lactation period