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9 Biosafety challenges and biological risks of engineered probiotic microorganisms Despite encouraging preclinical results, a substantial translational gap remains between experimental findings and the clinical deployment of engineered probiotics

Sample homogenate proteins (2,5 and 5 g/lane to SOD-1 and catalase, respectively) were separated by SDS-PAGE electrophoresis (15% polyacrylamide) according to literature-based methods and electrophoretically transferred to a nitrocellulose membrane by electroblotting (150 mA V, 2 h)

GGT1 mRNA (a) (n = 2 technical replicates representative of one experiment) and GGT activity (b) (n = 8 technical replicates from 3 independent experiments) in control (208 M cystine) medium were determined