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For relative expression analysis, the comparative 2 (Ct) method was applied to normalise IFNG expression to the endogenous EF1A reference and to express modulation as the relative FC to PBS controls

Exogenous NO application enhances antioxidant enzymes activity, limiting Cd absorption and promoting nutrient accumulation in tomato ( 2 S significantly improves plant resistance to Cd by enhancing the antioxidant defenses and essential nutrient absorption (Zhu et al., 2020a)

Feldstein AE, Werneburg NW, Canbay A et al (2004) Free fatty acids promote hepatic lipotoxicity by stimulating TNF-alpha expression via a lysosomal pathway

For gut fungal analysis, the independent samples t-test (SPSS 19.0 for Windows) was used to calculate the significant differences between the two groups