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doi: 10.1016/j.tiv.2011.12.002 11 ChenMLChenJSTangCLMaoIF

Concentrations of glutamate released following spinal cord injury kill oligodendrocytes in the spinal cord

The existing methods for phenotypic selection of genetically modified mammalian cells are mainly based on three types of phenotypic markers: an exogenous drug resistance gene that permits screening for positive cells, a fluorescent protein, such as GFP, that allows isolating positive cells by FACS (fluorescence-activated cell sorting) ( Recently, we developed a gene transfer-positive cell sorting system using membrane anchoring Twin-Strep-Tag ( Glutathione-S transferase (GST) is a multifunction enzyme that catalyzes the conjugation of glutathione to electrophilic compounds ( Results Design the membrane anchoring glutathione S-transferase fusion maker We use membrane anchoring GST molecule as the affinity tag to allow the transfection-positive cells to be isolated using GSH beads (Figure 1)

3.6 Gut microbiota composition analysis To explore the influence of TKP on the gut microbiota of constipated mice, 16S rRNA sequencing was conducted in the cecal contents