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The mice in groups 2, 3, and 4 were subcutaneously injected with 0.5 mL of scopolamine hydrobromide (2.5 mg/mL

We also tested the cleavage process in the presence of calcium, our results suggested that no obvious influence on the cleavage efficiency was found when the concentration of Ca 2+ is less than 5 mM, but the cleavage was dramatically impaired at 10 mM Ca 2+ (Supplementary Fig

In the STAIR XI meeting this point was made more explicit and recommended to be taken into account in the design of preclinical studies [25, 26]

A significant limitation of current studies on lactylation is their predominant reliance on mouse models that represent only specific pathological features, which do not fully reflect the actual conditions of patients brains