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Low B12 levels during pregnancy have been associated with an increased risk of low birth weight, preterm birth and miscarriage

The D-alanine substitution at position 2 is specifically directed at blocking the primary DPP-4 cleavage site, while the remaining substitutions prevent additional enzymatic attack at susceptible positions and reduce oxidative degradation during storage and in vivo

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Samples (0.0060.012 ml/well depending on the target protein) were loaded into a pre-cast mini-PROTEAN gel (Bio-Rad), run, and transferred in a min Trans-blot cell according to manufacturers instructions and consumables (Bio-Rad)