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Description

In the ALDEFLUOR assay (01700, STEMCELL Technologies, Vancouver, CA), functional stem cells were analyzed and isolated based on the fact that aldehyde dehydrogenase (ALDH) is a widely recognized key marker for breast CSCs 68

The reactive hyperaemia index (RHI) is calculated as the ratio of post-ischemic to baseline PWA using dedicated software (49)

We next sought to identify the site of EN25 modification on the GCL protein complex by performing LC-MS/MS analysis of tryptic digests from GCLC and GCLM labeled with EN25