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Description

Maxfield, L

Briefly, a radioactive probe containing the nucleotide sequence of the heavy-strand promoter of Tfam was prepared by annealing paired oligonucleotides with the sequences 5-TTTCCTCCTAACTAAACCCTCTTTAC-3 and 5-GTAGGCAAGTAAAGAGGGTTTAGTTA-3 and was labelled using - 32 P-labelled ATP (1.11 10 14 Bq/mmol