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Thymosin alpha-1, an essential component for a healthy immune system 3

Live/Dead staining was performed as described previously above and the cells fixed by adding cytofix/cytoperm (BD bioscience, USA)

2.7 Statistical analysis Statistical analysis was conducted using Statistica 13.3 (TIBCO Software Inc., StatSoft, Cracow, Poland), Graph Pad Prism 8 software (GraphPad Software, La Jolla, CA, USA), and PQStat 1.8.4 (PQStat Software, Poznan, Poland)