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The recombinant plasmid was then transformed into Escherichia coli BL21 (New England Biolabs, USA)

At that time, the cells were pelleted (400 g , 20 min) and resuspended in a staining solution containing 50 g/mL PI (81845, Sigma-Aldrich Chemie GmbH, Munich, Germany) and 50 U/mL RNase A (EURx, Gdansk, Poland) in PBS

Mechanism-dependent inhibition was also pointed out for CYP2A6 due to a marked decrease in IC 50 for the various cannabinoids but not for CYP2B6, where the IC 50 was similar (Yamaori et al., 2011a)