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For BSO treatment, cells were GSH starved for 48 h before washing and addition of EV-F3 and further 0.4 mM BSO (Sigma)

[PMC free article] [PubMed] [Google Scholar] 31.Ji Z, Liu G-H, Qu J

Results IGF-enriched pathways include activated cell cycle, DNA replication and repair and suppressed immune pathways As a first step to investigate associations of IGF axis components with immune factors, we analyzed effects of IGF-1 on gene expression in three PCa cell lines: two human, androgen receptor (AR)-positive 22Rv1 cultured from a xenograft established from primary PCa, and AR-negative DU145 derived from metastatic cancer

The difference is a modification to the molecule that extends how long it stays active in the body, giving a longer and more sustained growth-hormone pulse per injection