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The indicated plasmid combinations were used for the protein-protein interaction test according to the manufacturers instructions (Clontech, Phenotype characterization In vitro pollen germination was conducted in a Petri dish containing solid PGM [1% low melting point agarose (w/v), 18% sucrose (w/v), 0.01% boric acid (w/v), 1 mM CaCl 2 , 1 mM Ca(NO 3 ) 2 , and 1 mM MgSO 4 , adjusted to pH 7.5 using 0.2 M KOH] at 28 C, following a protocol modified from Boavida and McCormick (2007) 67

Here we used the SK-N-SH D human neuroblastoma cell line as a cell culture model to further investigate the involvement of TNAP in neuronal precursor proliferation and neuronal differentiation

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