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Phyllanthin nanoparticles, prepared with PLGA, acetone, and Tween via the nanoprecipitation method, were administered to a CCL4-induced liver fibrosis model

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The beads were washed extensively with lysis buffer, and bound proteins were analysed by SDS-PAGE and immunoblotting

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Finally, the results presented here provide useful information for understanding the catalytic mechanism and structure-function relationship of OsDHAR