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J774 cells are invaded for 1 h with E

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Equipment and Reagents Required but Not Provided Test Tubes Pipette capable of delivering 1 ml Graduated cylinder to measure 9 ml Schleicher & Schuell syringe filter units, disposable, cellulose acetate, pore size 0.2 m, Catalog Number F0303 Nickel(II) chloride hexahydrate (NiCl2 6H2O, Catalog Number 223387) or Cobalt chloride hexahydrate (CoCl2 6H2O, Catalog Number 202185), 0.3% (w/v) solution for enhancement of tissue stains Tris buffered saline (TBS), Catalog Number T5030, for washing Precautions and Disclaimer This product is for R&D use only, not for drug, household, or other uses

(G) Relative cell numbers for ZR-75-1 cells treated with 0.2 mM amino-oxyacetate (AOA) or under control or acidosis conditions