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Cells were cooled on ice, washed and scraped into buffer (1 mM EDTA, 100 mM sodium phosphate pH 7.5), and homogenized

Nov 7 2021;18(21)doi:10.3390/ijerph182111693

faecalis utilizes a bifunctional GCS-GS enzyme for glutathione biosynthesis, rather than the two-step pathway present in other bacteria and eukaryotes (38)