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The PTK hydrogel-treated group showed the highest ICRS macroscopic score of 11.3 (the score was 12 for native cartilage), which was 2.9 and 5.7 times that in the PMI hydrogel-treated group and the control group, respectively

Measurement of hydrogen peroxide production during enzyme reaction of FSP1 The content of H 2 O 2 during in vitro enzyme reaction of FSP1 was determined in aerobic or anaerobic conditions by using a hydrogen peroxide fluorometric assay kit (Elabscience, China), which takes advantage of H 2 O 2 reacting with the fluorescent probe in the presence of peroxidase to generate fluorescence intensity ( ex = 535 / em = 587 nm) that is proportional to the H 2 O 2 concentration range from 0.02 to 10 M

Overexposure of plants to Sub stress typically results in hypoxia, shortage of oxygen, or anoxia, a total absence of oxygen, which ultimately leads to tissue damage or plant death in the worst case (Loreti and Striker, 2020)

Jackson et al., 2003