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To validate macrophage activation, mouse bone marrow-derived macrophages were extracted and incubated with cancer cells, that have been treated with the nanoparticles

Through ACSL4 induction and provision of oxidizable PUFA substrates, M1 TAMs directly enhance the lipid-peroxidation machinery necessary for ferroptosis execution

They diverge in subsequent steps, where different reagents/conditions are applied to convert sulfenic acid to a thiol or sulfonic acid

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