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Freezing can cause the vitamins (especially riboflavin and perhaps dexpanthenol) to precipitate or the solution to phase-separate

Reportedly, the high catalytically active mitochondrial carbonic anhydrases (CAVA/CAVB) regulate the intercellular depot of bicarbonate ions and catalyze the rapid carboxylation of pyruvate and acetyl-co-A to acetyl-co-A and malonate respectively, which are the precursors of fatty acid synthesis and lipogenesis

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Early trials in the 2000s did not show meaningful weight loss versus placebo, and it never received FDA approval for that use