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Functional mtDNA mutations that impair oxidative phosphorylation and reduce ATP production markedly weaken the ability of cells to maintain energy homeostasis through endogenous mitochondria, thereby increasing their metabolic dependence on exogenous functional mitochondria [372]

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By detecting the appearance of the colored quinones on a TECAN infinite M200 absorption curves were recorded at different substrate molarities in a total volume of 200 l containing 50 mM sodium phosphate buffer at pH 6.0, 2 mM SDS and variable amounts of enzyme (Table S7) at 25 C