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Following collection, adipose tissue explants were rinsed in phosphate-buffered saline (PBS) (P4474, Sigma-Aldrich, Pozna, Poland) supplemented with 1% penicillin/streptomycin (P/S) (P4333, Sigma- Aldrich, Pozna, Poland) to prevent microbial contamination and then transferred into transport medium consisting of Dulbeccos Modified Eagles Mediumlow glucose (DMEM-LG containing 1000 mg/L of glucose) (D9443, Sigma- Aldrich, Pozna, Poland) supplemented with 1% P/S

Neuropeptides activate human mast cell degranulation and chemokine production

Funding We would like to thank for the support of Sub-project of Academician Zhong Shizhen Workstation, Joint cultivation project of Southwest Medical UniversityThe Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University (Osteopathy of Integrated Traditional Chinese and Western Medicine), and Innovation team project of The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University and the basic research project of the Applied Basic (2021ZKMS050) of Southwest Medical University for their support, 2020 Luzhou Science and Technology Plan Project Research on Body-Medicine Integration Driving Mechanism (Project No

This does not mean the treatment failed or that you are doing something wrong