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The number of neurons in the GCL and the thickness of the IRL (from the internal limiting membrane to the inner boundary of outer nuclear layer) were measured, as we previously reported 83

thaliana with the BLAST tool, and searched GhGPX9, 10 from another database ( The GhGPX genes were amplified by PCR (Fast Pfu DNA polymerase, Novoprotein) and the primers used for cloning and analysing the full-length CDS of GhGPX genes were designed by using Primer Premier 5 and their sequences were listed in Supplementary Table S1

These environmental factors are not confined to specific regions but are omnipresent, with notable occurrences observed in South Asia and Central America, as evidenced by studies conducted in Nicaragua, El Salvador, India, and Sri Lanka [41, 42]

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