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The production involves the combination of copper ions with short chains of amino acids, forming the copper peptide compounds

Concurrently, it is noteworthy that extracellular fatty acids (FAs) can enhance Gln metabolism levels and promote tumor cell proliferation

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For analysis of cells embedded in soft agar, cells (10 5 ) were suspended in 1 mL of 0.33% noble agar, seeded in the bottom of 15-mL polypropylene tubes, cooled for 5 min on ice, and then kept at 37 C in a humidified incubator in the presence of 5% CO 2 for 24 h72 h