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In darkness, opsin in photoreceptor cells covalently bind 11- cis -retinal

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These effects transcend any potential activities associated with the physiological (100 M) concentration of ascorbic acid added to the culture medium as a co-factor for prolyl and lysyl hydroxylases involved in collagen synthesis, since 8898% of ascorbate disappears from culture medium within 24 h 47 and we found the peroxyl scavenging activity of 100 M ascorbic acid was low (6 M TE)