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Wessels D, Lusche DF, Voss E, Kuhl S, Buchele EC, Klemme MR, et al

A commonly referenced low-dose starting point is: Around 1 milligram per kilogram of body weight More broadly, discussions around methylene blue dosing often highlight a low-dose window, since the compound appears to follow a hormetic response where smaller amounts can behave differently from higher concentrations

In Fmr1 knockout mice, GABAergic inhibitory function in the CA1 region of the hippocampus was impaired, as evidenced by reduced amplitude and frequency of eIPSCs, spontaneous IPSCs, and miniature IPSCs, along with significantly decreased expression of 2, 1, and GABAA receptor subunits in the hippocampus, which is associated with behavioral and cognitive deficits

doi: 10.1038/s41572-019-0069-0 4 SwensonKESwensonER