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Therefore, the average expression stability (M-value) was determined using the geNorm algorithm (19) in R Studio (version 2023.12.1 + 402, R version 4.3.2) with packages tidyverse and ctrlGene

About 520% of symptomatic gallstone patients undergoing cholecystectomy have choledocholithiasis and the incidence increases with age [180]

doi: 10.1507/endocrj.EJ16-0109 27 SaravananPChauW-FRobertsNVedharaKGreenwoodRDayanC

2.4 S-5-HT assay The S-5-HT (as well as all other laboratory) samples were collected at the Helsinki University Hospital Laboratory, where the S-5-HT protocol includes dietary restrictions for two days prior to the sample and collecting the samples between 8 10 am