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Pellets were thawed on ice for 1 h and lysed in 200 l 1% SDS in PBS supplemented with 1 protease inhibitors (cOmplete, EDTA-free Protease Inhibitor Cocktail, Roche, 11836153001) and shaken for 30 min at room temperature with 1:1,000 v/v benzonase nuclease (Sigma, E1014)

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The results indicate a significant decrease in reintubation rates, in incidence of atelectasis, and in mortality for patients treated with the regimen of heparin and N-acetylcystine [corrected] when compared with controls

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doi: 10.1016/j.tiv.2016.01.014 78 WangNZhouYFuCWangHHuangPWangBet al

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Nature Protocols 5 , 201208 (2010) Chuang, K

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