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Conditioned medium was collected and recipient HCT116 cells infected adding Polybrene (Merk, #TR-1003-G) (1 g L 1 )

We then used NMR and isothermal titration calorimetry (ITC) assays to validate the direct association of BRD4 with ASXL1

3.3 Mechanistic target of the rapamycin Particularly, when ACSL4 was overexpressed, mTOR phosphorylation greatly increased, and when ACSL4 was downregulated, mTOR phosphorylation significantly decreased