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The isolated cDNAs from the pool enriched in the fourth and fifth rounds were ligated into the pGEM-T-Easy vector (Promega), using DNA Ligation Kit Mighty Mix (Takara Bio), and cloned into DH5 competent cells

Thoughts on Getting Enough Vitamin B12 on a Vegetarian Diet Genes Mentioned Contents There arent a lot of things the nutrition world agrees on, but this may be one of them: vegetarians, and especially vegans, have a tough time getting enough Vitamin B12