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RMG-1 cells (obtained from Fisher Scientific, NC0992912) were cultured in Hams F12 Medium containing 10% (v/v) fetal bovine serum (FBS) and 10mM glutamine and maintained at 37C with 5% CO2

Notably, Arakeri et al

FIGURE 3 3.2.1 -L-glutamyl-4-nitroanilide (GPNA) GPNA, a glutamine analog synthesized by Esslinger, has been widely used to inhibit the glutamine transporter ASCT2 (Esslinger et al., 2005)