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The neurons were either immediately scraped into lysis buffer, to detect the entire surface pool (50 mM Tris-HCl, 150 mM NaCl, 2 mM EDTA, 0.5% IGEPAL, 0.5% sodium deoxycholate, 0125 mM PMSF protease inhibitor, and 1x protease inhibitor cocktail: #87786

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