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Western blot HEK-293T cells expressing hCC WT or the L68Q variant were washed twice with ice-cold phosphate-buffered saline (PBS) and lysed on ice using a freshly prepared ice-cold cell lysis buffer containing 50 mM Tris-HCl, pH 7.4, 100 mM NaCl, 50 mM -glycerophosphate, 10% glycerol (w/v), 1% NP-40 (w/v), 1 mM EDTA, 2 mM NaVO4, and a complete, EDTA-free, protein inhibitor cocktail (Roche Applied Science, Mannheim Germany) at 20 l per mL of lysis buffer

184 CDs and their derivatives, formed via structural modification of either primary or secondary face of parent CDs with aliphatic hydrocarbon chains, have been considered as potential excipients for different applications, including the production of nanospheres or nano-capsules of high loading capacity providing efficient targeting delivery

10.1038/nri2216 253 ZwellingL

OCT4/POU5F1 is required for NANOG expression in bovine blastocysts