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To the flask add 2.5 mL of hypophosphorous acid, close, boil gently but short of distillation for 10 minutes, then distill 1 mL into a test tube containing 1 mL of sodium hydroxide solution (1 in 50)

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Ann Rheum Dis 70:6067 Acknowledgements The authors would like to acknowledge Elettra Pignotti for her help with the statistical analysis, Maurizio Busacca for assistance in MRI evaluation, and Zimmer Biomet for providing kits and centrifuge