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Conventional redox-sensitive GFPs (roGFPs) are limited by undefined specificity and slow response to changes in redox potential

Environ Sci Pollut Res 29(10):1441814429 Nakamura SI, Suzui N, Yin YG, Ishii S, Fujimaki S, Kawachi N, Rai H, Matsumoto T, Sato-Izawa K, Ohkama-Ohtsu N (2020) Effects of enhancing endogenous and exogenous glutathione in roots on cadmium movement in Arabidopsis thaliana

Full oxidation of the sensor causes an increase of fluorescence at 395 nm and a corresponding decrease in 488 nm excitation while full reduction of roGFP2-iL leads to decreased fluorescence excitation at 395 nm and an increase at 488 nm

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