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Description

& Bianco, A

Histological examination Upon sacrifice, a small piece of liver tissue from each animal was collected and fixed immediately in 10% neutral buffered formalin

For histidine in Figure 5-3, three pK a s can be detected: the carboxyl group has a pK a = 1.82 the imidazole group has a pK a = 6.0, and the -amino group has a pK a = 9.17

At the end of the treatment the cells were collected by trypsinization and counted using Countess Automated Cell Counter from Life Technologies (Gaithersburg, MD, USA) according to the manufacturers instruction