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After 24 hours at 37C under anaerobic conditions, a sample of culture supernatant was collected and quenched by addition of formic acid to a final concentration of 0.1%

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The mechanism may be related to curculigoside significantly reducing AD-promoting factors (e.g., A1-42, p-tau) and increasing ferroptosis protective factors (e.g., GPX4, SLC7A11, GSH) in the hippocampus and cortex of AD mice, 574 suggesting that the natural compound curculigoside can be used as a promising therapeutic agent to improve AD by inhibiting ferroptosis